Journal: Molecular Medicine Reports

Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation

doi: 10.3892/mmr.2026.13828

Figure Lengend Snippet: STAT1 and TUBB4A expression levels are significantly correlated and upregulated in melanoma. (A) Analysis of data in the Gene Expression Profiling Interactive Analysis 2 database revealed a significant elevation of STAT1 expression in melanoma tissues. Quantification of (B) STAT1 and (C) TUBB4A mRNA levels in 31 paired melanoma and normal tissue samples from patients with SKCM demonstrated a significant upregulation of both STAT1 and TUBB4A expression in melanoma tissues. (D) Correlation analysis of STAT1 and TUBB4A mRNA levels in melanoma samples showed a significant positive correlation. Comparative analysis of (E) STAT1 and (F) TUBB4A mRNA expression in normal HEM and melanoma cell lines revealed significantly higher expression in the melanoma cell lines. *P<0.05 between groups; **P<0.01 compared with HEM cells. HEM, human epidermal melanocytes; STAT1, signal transducer and activator of transcription 1; TUBB4A, tubulin β4A; TPM, transcripts per million; SKCM, skin cutaneous melanoma.

Article Snippet: The melanoma cell lines A2058 (cat. no. CRL-3601), SK-MEL-1 (cat. no. HTB-67), A375 (cat. no. CRL-1619) and RPMI-7951 (cat. no. HTB-66), as well as normal human epidermal melanocytes (HEM; cat. no. PCS-200-013) and 293T cells (cat. no. CRL-3216) were obtained from American Type Culture Collection.

Techniques: Expressing, Gene Expression

Journal: Angiogenesis

Article Title: Exploiting porphyrin metabolism to inhibit angiogenesis

doi: 10.1007/s10456-026-10034-y

Figure Lengend Snippet: ALA promotes porphyrin overdrive in human microvascular EC a Intracellular porphyrins levels in HMEC treated with 5 mM ALA for 4, 24, and 72 h. b – c Fluorescence imaging and relative quantification of intracellular porphyrins (magenta) in HMEC treated with 5 mM ALA and controls. Scale Bar: 50 µm. n = 12 d Intracellular heme levels in HMEC treated with 5 mM ALA for 4, 24, and 72 h. e Mitochondrial activity of ALAS in HMEC treated with 5 mM ALA for 24 h (hrs). f – h Representative Western Blot images ( f ) and their quantification g – h of ALAS1 and HO-1 protein levels in HMEC treated with 5 mM ALA for 24 and 72 h. i – j Differential expression of heme-related genes in HMEC upon 24 h of 5 mM ALA treatment. The heatmap shown in ( i ) displays differences in gene expression levels, represented as fold change (FC) respect to not-treated (NT) cells. Volcano plot shown in ( j ) represents the differences in gene expression levels versus -log10(q value). The dotted line indicates the significance threshold of FDR q < 1% (-log10(q value) = 2). The plotted results represent mean values obtained from at least 5 individual biological replicates. k – l Extracellular levels of porphyrins ( k ) and heme ( l ) in HMEC after 4, 24, and 72 h of 5 mM ALA supplementation. Data are expressed as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. For statistical analyses, ordinary one-way ANOVA test with Tukey’s multiple comparisons ( a , d , g , h , k , l ) and parametric unpaired t test ( c , e ) were used. FC, fold change; NT, not treated controls; hrs, hours; ALA, 5-amminolevulinic acid

Article Snippet: Human adult dermal microvascular endothelial cells (HMEC-1, RRID:CVCL_0307) were purchased by ATCC, propagated in MCDB131 (Thermo Fisher Scientific, Waltham, MA USA, catalog n°10,372,019) supplemented with 10% heat-inactivated low-endotoxin FBS (GIBCO by Thermofisher Scientific, Waltham, MA USA, catalog n10270106), 10 mM GlutaMAXTM Supplement (Thermo Fisher Scientific, Waltham, MA USA, catalog n°35,050,061), 10 ng/mL Epidermal Growth Factor (Thermo Fisher Scientific, Waltham, MA USA, catalog n° PHG0315), 1 μg/mL Hydrocortisone-Water Soluble (Sigma Aldrich, St. Louis, MO USA, catalog n° H0396) and used up to passage 12.

Techniques: Fluorescence, Imaging, Quantitative Proteomics, Activity Assay, Western Blot, Gene Expression

Journal: Angiogenesis

Article Title: Exploiting porphyrin metabolism to inhibit angiogenesis

doi: 10.1007/s10456-026-10034-y

Figure Lengend Snippet: ALA inhibits in vitro angiogenesis on human microvascular EC ( a and b ) Proliferation of HMEC treated with increasing concentrations of ALA (500 nM, 0.1 mM, 5 mM) at various time points. Scale bar: 500 µm. n = 6 (c and d) Wound healing experiment of HMEC treated with increasing concentrations of ALA (500 nM, 0.1 mM, 5 mM) at various time points. Scale bar: 500 µm. n > 8 (e – h) in vitro tubulogenesis assay performed on 5 mM ALA-treated and control HMEC. Quantification of the total length ( f ) of the networks, number of nodes ( g ) and number of branches ( h ) are shown. Scale bar: 500 µm. Data are expressed as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. For statistical analyses ordinary two-way ANOVA test with Tukey’s multiple comparisons ( b , d ) and parametric unpaired t-test ( f – h ) were used. FC, fold change; NT, not treated controls; hrs, hours; ALA, 5-amminolevulinic acid

Article Snippet: Human adult dermal microvascular endothelial cells (HMEC-1, RRID:CVCL_0307) were purchased by ATCC, propagated in MCDB131 (Thermo Fisher Scientific, Waltham, MA USA, catalog n°10,372,019) supplemented with 10% heat-inactivated low-endotoxin FBS (GIBCO by Thermofisher Scientific, Waltham, MA USA, catalog n10270106), 10 mM GlutaMAXTM Supplement (Thermo Fisher Scientific, Waltham, MA USA, catalog n°35,050,061), 10 ng/mL Epidermal Growth Factor (Thermo Fisher Scientific, Waltham, MA USA, catalog n° PHG0315), 1 μg/mL Hydrocortisone-Water Soluble (Sigma Aldrich, St. Louis, MO USA, catalog n° H0396) and used up to passage 12.

Techniques: In Vitro, Control

Journal: bioRxiv

Article Title: Potent broad-spectrum antiviral activity of the marine natural product Plitidepsin

doi: 10.64898/2026.02.24.707815

Figure Lengend Snippet: A549 cells or IMR90 human lung fibroblasts were pretreated with 5 nM Plitidepsin for 2 hours, then infected with ( A - C ), recombinant IAV-GFP, ( D - F ) VSV-GFP, or ( G , H ) HCMV-GFP at MOI 5. At 24 hpi, ( A , D , G ) percentage of GFP-positive cells and ( B , E , H ) mean GFP fluorescence intensity were quantified by flow cytometry. ( C , F ) Viral titers in culture supernatants were determined by plaque-forming assay, respectively. Data represent mean ± standard deviation from two independent experiments in triplicate. Viral titers are expressed as PFU/ml. Statistical analysis was performed using unpaired Student t-test. Significance levels: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant. ( I-K ) A549 or IMR90 cells were treated and infected as described above. At indicated time points post infection ( I ) IAV NS1, ( J ) VSV glycoprotein (G), and ( K ) HCMV CCH2-DDG9 protein levels were assessed by Western blot analysis using GAPDH as loading control.

Article Snippet: The following cell lines were used in this study: human dermal fibroblasts (HDFs, ATCC, Cat. # PCS-201-012); human microglial cells (HMC3, ATCC, Cat. # CRL-3304); human cervical adenocarcinoma cells (HeLa, ATCC, Cat. # CRM-CCL-2); African green monkey kidney epithelial cells (Vero E6, ATCC, Cat. # CRL-1 586); human hepatocellular carcinoma cells (Huh-7D12, Sigma-Aldrich, Cat. # 01042712-1VL); human lung adenocarcinoma cells (A549, ATCC, Cat. # CCL-185); and human lung fibroblasts (IMR-90, ATCC, Cat. # CCL-186).

Techniques: Infection, Recombinant, Fluorescence, Flow Cytometry, Standard Deviation, Western Blot, Control